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CONGENITAL ADRENAL HYPERPLASIA
Aldosterone
Cortisol
DHEAS
Testosterone
Urinary 17 Ketosteroids
Urinary 17 Ketogenic steroids COLLAGEN VASCULAR DISEASE
ANA
ANCA ,
ds DNA (SLE )
Histone antibody
Jo1 (Myositis) antibody
RNP sm antibody
SCL 70 (Systemic Sclerosis )
SM antibody
SSA antibody
SSB antibody
U1sn RNP antibody
Centromere ab . DEEP VEIN THROMBOSIS /THROMBOPHILA
Anti Throbin III activity and antigen
APC resistance test
Cardiolipin IgG & IgM antibody
Factor-v leiden activity and mutation
Lupus anticoagulant (LAC)
Protein C activity and antigen,
Protein S activity and antigen DIABETES MELLITUS
C- peptide,
GAD 65
Glycosylated Haemoglobin (HBA1C)
Insulin,
Insulin antibody
Islet antibody ,
Lipid profile
Microalbumin,
Blood sugar (fasting / PP) DIABETES INSIPIDUS
ADH - Antidieuratic hormone,
Osmolality,serum,
Osmolality,urine HIRSUTISM
FSH
LH
Prolactin
Testosterone-Total
Testosterone - Free
DHT
Androsteindione (A4)
DHEAS,
SHBG (Sex Hormone Binding Globulin)
Free Androgen index INFLAMMATORY BOWEL DISEASE
MPO-ANCA
PR3-ANCA
ASCA IgG
ASCA IgA MULTIPLE SCLEROSIS PROFILE
CSF Index
CSF Protein Electrophoresis
IgG CSF
Myelin basic protein
Oligoclonal band MULTIPLE MYELOMA DIAGNOSIS
Bence Jones Protein
Beta 2 Microalbumin serum,
Fish for Del.13q
Immuno fixation(Qualitative)
Immuno fixation (Quantitative)
Immunoglobulines (IgG ,IgA ,IgM )
Kappa light Chains
Lambda light chins,
Protein Electrophoresis serum &urine,
Bence Jones Protein Immunofixation MYASTHENIA GRAVIS
Acetyl Choline Receptorantibodies
ASKA-Anti skeletal Muscle Antibody ORGAN TRANSPLANTATION
ABO+Rh grouping
CMV-IgG & IgM Avidity
HBV-VCA-IGM
HBV-Markers –DNA Detection
HCV –antibody - RNA Detection
HIV-DUO, Blot
HLA Typing
Varicella Zoster IgG,IgM
VDRL ,TPHA , FTA-Abs for Syphilis OSTEOPOROSIS
Acid Phosphatase With fraction
DPD Urine,
Osteocalcine
Phosphorous
Vitamin D3 PANCREATITIS
Lipase
Amylase PARATHYROID
PTH- Para Thyroid Hormone (Intct)
24 Hr Urine Calcium
25 Hydroxy Vitamin D3
Alkaline Phosphatase Total +Bone,
Calcium Total + Ionized,
Magnesium PORPHYRIA
PBG-Porphobilinogen Urine
ALA ,-Amino Levulenic Acid,Urine
PHEOCHROMOCYTOMA
Adrenaline, Serum /Urine,
Nor Adrenaline Serum/Urine
VMA Urine ,
Meta Nephrine Urine PITUITORY ADENOMA
Prolacin
ACTH
HGH
TSH
FSH,
LH
TSH PNEUMONIA
Adenovirus IgM
Chlamydia Pneumoniae IgM
Cold Agglutination
Coxiella Brunetii IgM
Legionella Pneumophilia IgM
Mycoplasma Pneumophilia IgM & IgA
Para Influenza 1,2,3 IgM
RSV IgM RICKETESSIAL INFECTION
Weil-FelIx Test SARCOIDOSIS
Serum ACE,
1,2,5 Dihydroxy Cholecalciferol (Vitamin D) SYSTEMIC VASCULITIS
ANA
ANCA-P / C,
C3
C4
CH-50
C-Reactive Protein SYSTEMIC LUPUS ERYTHEMATOSUS
Haemogram
ESR,
ANA
Ds DNA
Anti RNP,
CRP
Antibodies to SSA (RO),
Histone Antibody,
LE Cell
Anti Sm Ab,
Anti Cardiolipin Ab IgG / IgM
Antibodies To SSB (La)
Skin Biopsy
Urine Analysis,
Kidney Biopsy.
Complement Levels PANCREATIC MARKER PROFILE
CEA
CA 19,9
GASTRIN
INSULIN THYROID MARKER PROFILE
Thyroglobulin
Calcitonin
NSE
CHORIOCARCINOMA
HCG
AFP, ANAEMIA
Abnormal Haemoglobin Studies
Acidified Hams Test
Cold aggulitinin
Cooms Direct
Cooms Indirect
Cytogenics For Fanconi’s anaemia
Cytogenics For B Thalassaemia
Erythropoetin
Ferritin
G6PD
Haptoglobulin
Intrinsic Factor antibody
Iron studies
Meth Haemoglobin
Osmotic Fragility
Parietal Cell antibody,Serum
Plasma Hb,
RBC Folate
Reticulocyte Count
Rh Antibody
Serum Folate
Sickling Test
Vitamin B12 TUBERCULOSIS
ADA(Adenosine deaminase )
AFB –stain (ZN stain)
Tuberculosis by PCR
Tuberculosis by TMA
Tuberculosis ,culture (LJ method)
Tuberculosis –IgA ,IgM and IgG
Tuberculosis culture (Rapid) HEPATITIS B
HBsAg
Anti HBsAg
HbeAg
Anti HBC Ag (Total)
Anti HBc Ag IgM
Anti HDV
HBV – DNA detection
HBV –DNA Quantification
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| Test Description | Sample | Sample Instruction | Store At | Method | Reporting Time | Test Fees | General Information | | Allergy – Food Screeening1 (Peanut,Hazelnut ,Brazil nut ,Almond ,Coconut | Serum | 3 ml serum | R | CLIA | 10th day | 2300 | Specific allergen screen | | Allergy – Food Screening 3 Wheat, Oat, Corn, seed, Buckwheat) | Serum | 3 ml serum | R | CLIA | 10th day | 1900 | Specific allergen screen | | Allergy – Food Screening 5 (Egg white, Milk, codfish, wheat, peanut, soybean) | Serum | 3 ml serum | R | CLIA | 10th day | 2650 | Specific allergen screen | | Allergy – Food Screening 6 (Wheat, Rice, Sesame seed, Backwheat, Soyabean) | Serum | 3 ml serum | R | CLIA | 10TH day | 2400 | Specific allergen screen | | Allergy - Food Screening 7 ( Egg White, Milk, Wheat, Rice, Peanut, Soyabean) | Serum | 3 ml serum | R | CLIA | 10th day | 2750 | Specific allergen screen | | Allergen – Flour Dust | Serum | 3 ml serum | R | CLIA | 9th day | 1000 | Specific allergen screen | | Allergen – Pork | Serum | 3 ml serum | R | CARLA | 9th day | 1000 | Specific allergen screen | | Anaemia Profile (CBC, Reticulocyte count., G6PD Osmotic fragility Hapatoglobulin, Abnormal Hb studies, DCT | Blood. Serum | 5 ml EDTA Blood
3ml Heparinised
3 ml serum | R | Cell counter
EP
Biochemical
ELFA | 4th day | 2200 | Clinical History is required | | Androgen Profile ( Testo – total & Free, SHBG, DHT, FAI, Androstenedione ) | Serum | 5 ml serum | R | RIA / CLIA | 6th day | 2900 | Clinical History is required | | Apolipoproteins Profile - (Apo A1 , Apo B, Lp (a) | Serum | 4 ml serum | R | Turbidimetry | 2nd day | 1200 | 12 – 14 hours fasting required | | Arthritis Profile ( CBC , RA,CRP,Uric acid , Urine RE ,ASO , Electrophoresis | Blood
Serum
Urine | 3 ml EDTA Blood
3 ml SERUM
10 ml Urine | R | Turbidimetry
Biochemical
EP
Microscopy | 2nd day | 1250 | | | Auto Immune Liver Disease Profile ( ASMA , AMA, LKM1 ,ANA ) | Serum | 3 ml serum | R | IF | 7th day | 2400 | See individual tests | | BOH – Bad Obstetric History Profile ( ANA , APLA IgG/IgM,ACA IgG/IgM ,LAC,TSH & Torch IgG/IgM | Serum
Plasma | 3 ml Serum
2 ml Citrated Plasma | R | CLIA
ACM
ELISA | 2nd day | 4150 | Plasma should be platelet poor needs to be well preserved. | | Bone Profile (Calcium + Phos +ALP +Proteins + Uric acid) | Serum | 3 ml serum | R | Biochemical | Daily | 310 | Collect without Tourniquet | | Breast Marker Profile ( CA 15.3 , CEA) | Serum | 4 ml serum | R | CLIA | 4th day | 1350 | Clinical History is required | Cancer Detection Profile – Female(AFP,HCG,CA125,CA19.9,CA15.3,
CEA Calcitonin ,Thyroglobulin,PAP smear | Serum
Smear | 5 ml serum
Pap smear | R | CLIA
M | 5th day | 5000 | Clinical History is required | | Cancer Detection Profile – Male ( AFP , CEA, HCG , Calcitonin , Thyroglobulin,PSA,CA15.3,CA19.9) | Serum | 5 ml serum | R | CLIA | 5th day | 4400 | Clinical History is required | | Cardiac Injury Profile ( LDH , CPK Total , CPK –MB, SGOT ) | Serum | 3 ml serum | R | CLIA | Daily | 550 | See individual tests | | Cardiac Risk Profile ( Homocysteine , LP(a) ,D-Dimer , CRP , LDL Direct and Apolipoproteins A1/ b) | Serum | 3 ml serum | R | CLIA
Biochemical | Next Day | 2700 | See individual tests | | Chemistry – 15 ( FBS , Ca , P, Cholesterol, Uric acid, Bilirubin T&D, Creat , ALP ,CPK , SGOT, SGPT, LDH , TGL , Proteins) | Serum | 900 | R | Biochemical | Daily | 1100 | 12 – 14 hours fasting required | | Diabetes Profile, Mini ( FBS, Cholesterol, HbA1c, Micralbumin) | Serum
Blood
Urine | 2 ml serum
2ml EDTA
2ml Urine | R | Biochemical | Daily | 450 | See individual tests | Diabetes Profile , Maxi( CBC,FBS PPBS,Cholesterol,TGL,Creat, Electro, Urine RE, Insulin , C-peptide , HbA1c ,
Microalbumin) | Serum
Blood
Urine | 2 ml serum
2ml EDTA
2ml Urine
2ml F.plasma | R | Cell counter
CLIA
Biochemical | Daily | 1600 |
See individual tests | | DIC (Coagulation) Profile (CBC, BT CT,PT,PTT ,Fibrinogen, D-Diamer ) | Blood
Plasma | 3 ml EDTA
2ml plasma | R | ACM
Cell counter | Next day | 1380 | See individual tests | | Endocrine Profile – Female ( FSH LH,PRL,E2,TSH) | Serum | 3 ml serum | R | CLIA | Daily | 1050 | See individual tests | | Endocrine Profile – Male ( FSH , LH, PRL , Testosterone ,TSH ) | Serum | 3 ml serum | R | CLIA | Daily | 1250 | See individual tests | | Fever Profile ( Maxi) (CBC , Urine RE , MP , SGPT , Widal ,Aerobic blood Culture) | Blood
Serum
Urine | 3 ml EDTA
3 ml serum
10 ml Urine | R | Cell counter
Biochemical |
Daily | 490 | See individual tests | | Fever Profile ( Mini) ( CBC , Urine RE ,ESR , MP , Widal ) | Blood
Serum
Urine | 3 ml EDTA
3 ml serum
10 ml Urine | R | Cell counter
Biochemical | Daily | 380 | See individual tests | | Haemophilia Profile ( APTT , Factor V111 , Factor 1X ) | Plasma | 3 ml Citrated Plasma | R | Coagulation | 4th day | 2400 | See individual tests | | Hypertension Profile (CBC, Urine RE, FBS , BUN. Creatinine, UAC, Electrolytes, Lipid profile) | Blood
Serum
Urine | 3 ml EDTA
3 ml Serum
10 Urine | R | Biochemical
Cell counter
Micrscopy | Daily | 800 | | | IBD Profile ( ANCA ,P ANCA , ASCA IgG & IgM ) | Serum | 4 ml serum | R | ELISA | 5TH day | 2500 | | | Immunoglobulin Profile( IgG , IgA , IgM ) | Serum | 3 ml serum | R | N | Next day | 900 | Age & Sex mentioned | | Infertility Profile ( Male ) ( CBC , FBS ,PPBS, BUN ,Urine RE, Semen RE, FSH , LH ,PRL , Testo , ASAB, Chlamydia IgG/IgA | Blood
Serum
Urine
Semen | 2 ml EDTA
4 ml serum
2 ml Fluoride
Urine & Semen | R | Cell counter
ELISA
CLIA
Microscopy |
4th day | 2300 | Age & Sex mentioned | | Infertility Profile (Female ) ( CBC, FBS, PPBS,BUN , Urine RE, Blood Gp +Rh , FSH LH ,PRL , TSH , ASAB , Chlamydia IgG/IgA | Serum
Blood
Urine | 2 ml EDTA
3 ml serum
2 ml Fluoride
Urine | R | Cell counter
ELISA
CLIA
Microscopy | 4th day | 2200 | Age & Sex mentioned | | Lipid Profile ( Cholesterol ,HDL ,LDL, VLDL , TGL ) | Serum | 3 ml Serum | R | Biochemical | Daily | 300 | 12 – 14 hours fasting required | | Liver Profile ( Bili T& D , SGOT , SGPT,ALP,Proteins ) | Serum | 3 ml serum | R | Biochemical | Daily | 300 | Test assesses the functional activity of the Liver) | | Liver Marker Profile ( AFP , CEA ) | Serum | 3 ml serum | R | CLIA | Daily | 700 | | | Metabolic Disorder Profile ( Includes 101 different analytes to diagnose 22 different metabolic disorders) | Urine | Spot Urine or
Wattman filter paper –3 dipped in urine and air dried | R |
GCMS | 20th day | 9000 | Wattman filter paper –3 dipped in urine and air dried is preferred | | Oesophageal Marker Profile ( CA19.9 ,CEA , SCC ) | Serum | 4 ml serum | R | CLIA
ELFA | 10th day | 2500 | | | Pancreatic Marker Profile ( CEA , CA 19.9 , Gastrin , Insulin ) | Serum | 4 ml Serum | R | CLIA
ELFA | 5TH day |
2250 | | | PCOD Profile ( FSH , LH, PRL , Testo , Insulin F & PP , FBS ,PPBS ) | Serum | 2 ml serum
2 ml Fluoride | R | CLIA
Biochemical | Daily |
1500 | Mention Age , LMP , Sex | | Pituitary Marker Profile ( HGH , ACTH , FSH , PRL , LH , TSH ) | Serum | 4 ml serum | R | CLIA | 3rd day | 1900 | ACTH is a labile analyte ,
Please collect before 12 noon in resting condition . | | STD Profile ( HIV –Duo , HSV 1 &2 IgG/IgM ,VDRL ,TPHA , Chlamydia IgG/IgA | Serum | 4 ml serum | R | ELISA
Agglutination | 5th day | 1500 | | | Stomach Marker Profile ( CEA , CA 19.9 ,CA 72.4 ) | Serum | 4 ml serum | R | CLIA
ELFA | 4th day | 2350 | | | Thyroid Marker Profile ( Thyroglobulin , Calcitonin , NSE ) | | 4 ml Serum | R | CLIA
ELISA | 4th day | 3000 | Detailed Clinical History is required | | Typhoid Profile ( CBC ,Urine RE , Widal , ) | Blood
Urine | 3ml blood
Urine | R | Cell counter
Microscopy | Daily | 280 | See individual test | | Thyroid Profile – Total ( T3 ,T4, TSH BY CLIA ) | Serum | 3 ml serum | R | CLIA | Daily | 350 | See individual test | | Thyroid Profile –FREE ( FT3 ,FT4, TSH BY CLIA ) | Serum | 3 mlserum | R | CLIA | Daily | 350 | See individual test | | Tuberculosis Antibody Profile ( TB- TgG, IgM ,IgA ) | Serum | 3 ml serum | R | ELISA | Daily | 900 | Detailed Clinical History is required | | Willsons Disease Profile (CBC , Ceruloplasmin , Serum Copper , 24 Hrs Urine copper, Proteins , SGOT ,SGPT, Bilirubin , RBS | Serum
Blood
Urine | 4 ml serum
2 ml EDTA
24 hrs Urine | R | Biochemical | Next Day | 1500 | See individual test | |
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ROUTINE 24 HOUR URINE COLLECTION The 24 hour urine specimen is to be submitted in a chemically clean, properly labeled urine container. Patients should not be allowed to submit urine specimen in their own “clean” jars. Metro lab adds required preservatives or supplies the proper preservative with the container. Patient is provided with written instructions for urine collections. Recommended patient instructions for 24-hour urine collections. To the patient: Follow these instructions in collecting your 24 hour urine specimen. You will find it more convenient to void (urinate) into the smaller container provided and transfer the urine in to the larger collection container. Do not add anything but urine to the container and do not pour out any liquid or powder that may already be in the collection container. These substances may cause burn if touched. The collection container should be kept refrigerated through out the collection period. Upon arising in the morning, urinate into the toilet, emptying your bladder completely do not collect the sample. Note the exact time and print it to the container label. Collect all urine voided subsequently for 24 hours in the container provided. All urine passed during the 24 hour time period (day or night) must be saved. Urine passed during bowel movements must also be collected. Refrigerate the collected urine between all voiding or keep it in a cool place. At exactly the same time the following morning void completely again (first time after awakening), and add this sample to the collection container. This completes your24 hour collection Take the 24 hour specimen to the physician’s clinic /laboratory as soon as possible, maintaining the cool temperature in transit by placing the specimen in a portable cooler or insulated bag. For the physician/laboratory/ collection centre: When aliquots are required with preservative, use only the preservative requested. If only a portion of the 24-hour collection is submitted to the lab, be sure to measure the entire 24 hour volume and record the total amount in milliliters (ml) on the test requisition form for laboratory use. When the lab requires a pooled, well –mixed specimen (collected in more than one container) or a measured aliquot for an individual test, provide the specimen prepared exactly as stated in the specimen requirement submit this to metro lab as soon as possible. In case if the test request is for two parameters requiring 24 hour urine collection with different preservative requirements then follow the below mentioned procedure: First 24 hour urine collection (HCl Preservatives ) Urinate in the usual manner on awakening, making sure to empty your bladder completely. Do not save this urine, but you must record the date and time of this first urination. Example: 13/01/2004 7:30 AM All urine passed during the remaining 24 hour period must be collected in this first container, labeled “HCL Preservative”. Urine passed during bowel movements must also be collected. Urine may be collected in another clean container and then carefully poured into the first 24 hour collection container.
Second 24- Hour Urine Collections (No Preservative) After the first 24 hours collection procedure is completed. For second collection start from step 1 to step 4 as given in first 24 hours collection accept the urine must be collected in a container without preservative. Labeled “No Preservative”
SPECIAL URINE COLLECTION PROCEDURES Preservatives for 24Hour Urine Collections | TEST | PARTICULARS | QUANTITY | | 17-keto steroid | HCl | 10-15 ml-50% | | 17-OH corticosteroid | Glacial acetic acid | | | 5-HIAA | HCl | 10-15 ml-50% | | Aluminum | Boric acid | 15 gm | | Adrenaline(Epinephrine) | Glacial acetic acid | 15 ml | | Aldosterone | Boric acid | 15 gm | | Calcium | HCl | 10 ml | | Copper | Thymol | 10 gm | | Cortisol | Boric acid | 15 gm | | Creatinine | Boric acid | 15 gm | | E-3unconjugated Estriol | Boric acid | 10 gm | | Metanephrine | Glacial acetic acid | 15 ml | | Nor-adrenaline | Glacial acetic acid | 15 ml | | Oxalate | HCl | 10 ml | | Phosphorous | HCl | 10 gm | | Protein | Boric acid | 10 ml | | Urea | HCl | 10 ml | | Uric acid | Boric acid | 15 gm | | VMA | HCl | 12 ml-25% | No preservatives to be added for the following: Urinary sodium
Urinary potassium
Urinary chloride |
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Coagulation assays (For PT, APTT and Fibrinogen) - Confirm if patient is taking any medicines/drugs. If yes then note down the names and doses.
- Confirm if patient is suffering from any other disorder like Thrombosis etc.
- One pair of sodium citrate (3.2%) should be mixed with 9 parts of whole blood.
- For sodium citrate tube of 2ml 1.8ml of blood(Up to the mark given on tube)
- For sodium citrate tube of 3ml2.7ml of blood(Up to the mark given on tube)
- After collecting the venous blood in the sodium citrate tube, mix it by gently inverting the tube 3 to 4 times
- Spin the sample at 3500 rpm for 20 minutes to separate the clear plasma.
- Separate the clear plasma in a plane plastic vial and again centrifuge for 15 minutes.
- Immediately pack the separated plasma in the ice pack and dispatch.
- If to be processed after 6 to 8 hours then store in the freezer at -20C.
If sample is from outstation then frozen plasma- containing vial must be packed in the sample Styrofoam box in dry ice. Collection time should be mentioned on the respective vial. Coagulation assays (For factor assays, Activity assays and Lupus anticoagulant. Platelet poor plasma (PPP) is must for assays The obtained plasma (by following the first five steps mentioned above) must be centrifuged again at 3500rpm for 15 minutes. The supernatant (PPP) should be transferred to properly labeled plastic vial. For factor assays like Factor V, Factor VIII, Protein C and S activity, citrated plasma should reach at metro lab in FROZEN state (refer the step number 8 and 9 mentioned above) Note: - If sample quality is unsatisfactory then repeat sample can be asked. |
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